Questions
Fig 2AB. To simplify study of these effects, the investigators performed in vitro (cell free) assays in which no ROS (superoxide, hydrogen peroxide) would be produced. They studied two different KATs, p300 (A) and CBP (B) using HeLa cell core histones, with 100 μm plumbagin (lanes 5–7 in A and B) either in the presence or absence of NAC, DTT, and another reducing agent Trolox (lane 8). Interpret the results. What part of NAC and DTT appear important in their effects on plumbagin inhibition.
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