Thermodynamics of the GTP-GDP-operated Conformational Switch of Selenocysteine-specific Translation Factor SelB. Alena Paleskava, Andrey L. Konevega and Marina V. Rodnina . The Journal of Biological Chemistry, 287, 27906-27912 (2012).  doi: 10.1074/jbc.M112.366120  .

c.   What is the most logical control experiment that you would perform?  How would you correct the experimental derived enthalpy changes for the those see in the control case?

answer:  inject ligand (GTP, GDP, etc) into the sample cell containing just buffer solution (which is also placed in the reference cell.  Subtract the DH for the dilutions from the DH for the experimental DHs.

Navigation: