TATA Box Binding Protein 


The TATA Box Binding Protein is a transcription factor that binds to the minor groove of an eight base pair segment of DNA allowing RNA Polymerase to bind and begin transcription.  The Base Pairs are characterized by a redundant TATA pattern.

Spacefill Model Of protein. Notice: Saddle-like shape. Concave "underside" distorts DNA significantly when bound.

Backbone and sidechains of TATA Box Binding Protein

View DNA-The protein binds to the minor groove of the DNA

Cartoon, Hbonds and Wireframe of TATA Box Binding Proteins Bound to Minor Groove of DNA.  Note: Anti-Parallel Beta Pleated Sheets in Protein Binding Site.  The Protein-DNA uses an induced fit mechanism

Binding site residues form a concave binding pocket for minor groove of DNA to bind

Binding Amino Acids along with spacefilling model of minor groove and interacting base pairs

Hydrophobes-Notice High Hydrophobicity in Binding Site.  TBP-DNA Complex is characterized by 74% Hydrophobicity within binding site.

Secondary Structure of the Binding Pocket showing antiparallel Beta-Pleated Sheets in Binding Site

Specific Binding Amino Acids in Binding Site.

Nikolov, D.B., Chen, H., Halay, E.D., Hoffmann, A., Roeder, R.G., & Burley, S.K. (1996). Crystal structure of a human TATA box-binding protein/TATA element complex. 93, 4862-4867.