Mechanism of auxin perception by the TIR1 ubiquitin ligase

Wire Frame in chains' colors
Three chains in the protein: Chain A - gray, Chain B - green, Chain C - red

Cartoon
Secondary structure of protein alpha helixes and beta sheets

Wireframe Auxin + Backbone Protein
Naphtalene-1-acetic acid (spacefill), a synthetic auxin (a mimetic of the most important auxin, indole acetic acid) and the backbone of protein

Temperature presentation of Protein with Auxin
The more mobile, "warmer" segments are colored red, progressing to more immobile blue fragments. NLA in spacefill.

Auxin interaction with protein
Chain B amino acids-green: HIS78, ARG403, SER438, SER462, GLU487, Chain C amino acids-red: PRO7, and TRP 5 interacting with NLA.

Solvent surface
Protein and Auxin with
water (1.4 A) around the molecule.

MEP for protein
T
he Map of Electrostatic Potential (MEP) shows the charge distribution (δ+and + charges which show as blue, and the δ- and - charges which show as red) on the atoms of the macromolecules.  Black spacefill is NLA.

Tan, X.,  Calderon-Villalobos, L.I.A.,  Sharon, M.,  Zheng, C.,  Robinson, C.V.,  Estelle, M.,  Zheng, N. (2007) Mechanism of auxin perception by the TIR1 ubiquitin ligase Nature 446: 640-645

 

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