CH 105 - Chemistry and Society

Alcohol: GABA Channel Activation and Modulation

Animation 1. Neurosteroid Binding Exposes a Phosphorylation Site (Model A). This animation shows one model for how neurosteroids may increase Cl flux through a GABA-A receptor. Binding of the neurosteroid (ALLO) allows a protein kinase C (PKC) phosphorylation site to become accessible. Phosphorylation of the channel increases flux through the channel, allowing more Cl ions to flow through the channel in the presence of GABA than when the channel is activated by GABA without prior phosphorylation. The top of this animated gif shows how the channel, the ligands, and G protein-activated PKC may be interacting at the plasma membrane. The bottom of the animation shows how these interactions affect the current trace recorded using electrophysiological techniques. In the current trace, the size of the signal is smaller in the absence of neurosteroid compared to the size of the signal after exposure to neurosteroid and subsequent phosphorylation of the channel.

Citation: J. Tasker, Regulation of GABA receptor activity by neurosteroids and phosphorylation. Sci. STKE 2004, tr4 (2004).

2004 American Association for the Advancement of Science