Concepts of Biology (BIOL115) - Dr. S.G. Saupe (ssaupe@csbsju.edu); Biology Department, College of St. Benedict/St. John's University, Collegeville, MN 56321

  Study Guide & Review Sheet for Enzymes

I. Goal: The goal of this unit is to develop a basic understand of the importance of enzymes and how they function.

II. Learning Objectives: Upon completion of this unit you should be able to:

1. explain why enzymes are important for life.
2. describe the lock and key and induced fit models for enzyme activity.
3. explain the difference between coenzyme and cofactor. Give examples of each.
4. describe and explain the influence of each of the following in the rate of an enzyme-catalyzed reaction: pH, temperature, enzyme concentration, substrate concentration, competitive and non-competitive inhibitors.
5. explain how enzymes lower activation energy.
6. describe ways to increase the rates of chemical reactions. Which do living systems use?
7. describe the reactions catalyzed by tyrosinase, catalase, rennin, amylase, and bromelin.
8. describe the ways that enzymes regulate the rate of metabolism
9. explain what is meant by Km, Vmax and how these values are calculated (not on exam)

III. Reading(s):

• Purves et al, Ch 6 (pp 144 - 153)
• Spuds McSaupe Plays with Peroxide
• Gink & Go are Mad as Hatters

IV. Personal Interest: Quickly skim the assigned chapter, unit goal, learning objectives and web site notes. Write down any areas of particular interest to you.

V. Warm-up: Describe any personal experience that you have had with this topic. This could include previous work in high school, last semester in concepts, or in a non-academic setting. If you've had absolutely no previous experience with the topic, then write down how this topic may impact your daily life. Look through the objectives; do you know the answer to any of them?

VI. Activities:

1. prepare written answers to the objectives
2. write a dialog, like Gink & Go, using as many of the terms in the chapter as possible
3. prepare a concept map for this material (see one given in class)
4. answer the questions at the end of the chapter (p. 152-3)
5. Visit the text website.  View the simulations and other materials, answer the on-line quiz questions.
6. Check out the Spuds McSaupe worksheet
7. Check out the Gink & Go dialog about the Mad Hatter
8. in your notebook, sketch and explain the following graphs:

• product formation vs. time
• number of molecules vs. energy per molecule.
• reaction rate vs. substrate concentration
• reaction rate vs. enzyme concentration;
• reaction rate vs. pH
• reaction rate vs. temperature
• Lineweaver-Burk plot (not on exam)

7. Define the following terms:

VII. Using Other Resources: What have you learned about this topic from other resources including periodicals, AV material, web sites, people outside of class, etc.? Write down something you've learned about this topic from another source.

VIII. Extensions:
    Explain why hydrogen peroxide foams when you place a drop of blood in it. If you heated the blood before putting it into the peroxide would it still foam up? What if you froze the blood first and then thawed it? Would the peroxide foam if the blood drop were first treated with a mercury salt?

IX. Questions: After completing the activities, write any question(s) that you still have concerning the objectives or that pertain to related areas

X. Application: Write one or more ways in which you can immediately apply this information to your daily life.

XI. Assessment: Meet with one or more members of the class to help assess that you have attained the objective and to help deepen your understanding of the material.