|Plant Physiology (Biology 327) - Dr. Stephen G. Saupe; College of St. Benedict/ St. John's University; Biology Department; Collegeville, MN 56321; (320) 363 - 2782; (320) 363 - 3202, fax; firstname.lastname@example.org|
Determining the Sucrose Concentration of Sugar Maple Sap by the Refractive Index Method
The concentration of sucrose in a sample can be determined by measuring the refractive index of the sample. This measurement is based on the principle that the index of refraction of a solution containing sugar is proportional to its concentration. We will use an Atago Hand Sugar Refractometer to measure sugar concentration. (Note: other types of refractometers will work equally well, but in order to obtain a concentration value, the user needs to refer to standard tables or run a standard curve with sucrose solutions of known concentration).
01/07/2009 � Copyright by SG