|Plant Physiology (Biology 327) - Dr. Stephen G. Saupe; College of St. Benedict/ St. John's University; Biology Department; Collegeville, MN 56321; (320) 363 - 2782; (320) 363 - 3202, fax; firstname.lastname@example.org|
Techniques to View Stomata
I. Making an Epidermal Leaf Peel
Note: The lower epidermis generally strips more easily than the upper epidermis. Some species are more difficult to strip than others. Vicia faba (broad bean) and Zebrina sp. (Wandering Jew) are especially easy to peel.
Paint clear nail polish on the leaf surface and allow it to dry. To remove the leaf "cast", use a piece of clear cellophane tape which can then be taped to a microscope slide for viewing. In some cases, it is even possible to apply the tape directly to the leaf, rub it down and then remove it and transfer it to a microscope slide.
01/07/2009 © Copyright by SG