|Plant Physiology (Biology 327) - Dr. Stephen G. Saupe; College of St. Benedict/ St. John's University; Biology Department; Collegeville, MN 56321; (320) 363 - 2782; (320) 363 - 3202, fax; firstname.lastname@example.org|
Preparation: Stomata Lab
1. Define abaxial and adaxial.
2. Sketch and label a dicot-type
3. Sketch and label a
monocot-type stomatal apparatus.
4. The following diagram
represents guard cells/stomata in a field of view in the microscope.
Assume that the width of the field is 300 mm.
Calculate (a) area of the
field; and (b) stomata mm2.
5. What can you conclude from the following data extracted from Woodward, FI (1988) Plants Today, July-August.
III. Lab Materials
Broad beans (.3 wk, 1 per person)
Greenhouse plants (i.e., Wandering Jew, maize or other grass)
50 mM potassium phosphate buffer pH 6.0 (880 mL 50 mM KH2PO4 and 120 mL of 50 mM Na2HPO4)
50 mM KCl
10 uM Abscisic acid (FW 264.3; dissolve .0026 g in acetone make up to 100 mL)
1 M salt or sucrose in dropper bottle
60 x 15 mm petri dishes (4 per pair)
microscopes fitted with ocular micrometers
aluminum foil � small squares to cover petri dishes
tool kits (with tape, ruler, marking pen, razor blades)
01/07/2009 � Copyright by SG