Learning Goals/Objectives for Chapter 5D: After class and
this reading, students will be able to
- describe general mechanisms of how a gene for a given
protein might be negatively and positively regulated at the
level of gene transcription;
- describe the structure/function/role of promoters, response
elements, RNA polymerase, transcription factors, nucleosomes,
histone proteins, epigenetic modifications of DNA in gene
transcription;
- explain the differences (structural, Kds) between specific
and nonspecific binding of a ligand to a macromolecule, at the
structural level;
- describe the structural features of both proteins and DNA
that result in specific and nonspecific binding;
- describe and give examples of how post-translational
modifications of proteins and epigenetic modifications of DNA
can alter gene expression;
- explain how the apparent Kd for a protein binding to DNA can
be altered by the presence of another protein bound to DNA at a
proximal site
- describe the basis of RNA interference in gene expression
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