Chymotrypsin Activity in Organic Solvents

Solvent Structure kcat/Km (M-1min-1) relative ratio
kcat/Km
H2O bound to enzyme (%, w/w)
Octane

63 15000x 2.5
Toluene

4.4 1000x 2.3
Tetrahydrofuran

0.27 175x 1.6
Acetone

0.022 5.5x 1.2
Pyridine

<0.004 1x (.004) 1.0