Chymotrypsin Activity in Organic Solvents
Solvent | Structure | kcat/Km (M-1min-1) | relative ratio kcat/Km |
H2O bound to enzyme (%, w/w) |
Octane | 63 | 15000x | 2.5 | |
Toluene | 4.4 | 1000x | 2.3 | |
Tetrahydrofuran | 0.27 | 175x | 1.6 | |
Acetone | 0.022 | 5.5x | 1.2 | |
Pyridine | <0.004 | 1x (.004) | 1.0 |