Chymotrypsin Activity in Organic Solvents
Solvent | Structure | kcat/Km (M-1min-1) | relative ratio kcat/Km |
H2O bound to enzyme (%, w/w) |
Octane |
|
63 | 15000x | 2.5 |
Toluene |
|
4.4 | 1000x | 2.3 |
Tetrahydrofuran |
|
0.27 | 175x | 1.6 |
Acetone |
|
0.022 | 5.5x | 1.2 |
Pyridine |
|
<0.004 | 1x (.004) | 1.0 |