Chymotrypsin substrate cleavage, 25
o
C, pH 7.9
kinetic constants
Acetyl-Tyr-Gly-amide
Acetyl-Tyr-O Ethylester
Ester/Amide
k
cat
(s
-1
)
0.50
193
390
Km (M)
0.023
0.0007
0.03
k
cat
/Km (M
-1
s
-1
)
22
280,000
12,700