Partition of Solutes into Hydration Sphere of BSA

Table: Summary of Partition Coefficient (K_P = m^{hydration
sphere}/m^bulk) for urea and GuHCl effects on protein stability for BSA

Solute K_{P N} K_{P D} Select partition into % surface polar backbone N,D % surface charge N,D % ΔSA N<=>D: backbone, charge Effect on N<=> D

KCl 1 1 neither 13,13 19,9 13,4 none

urea 1.1 1.1 hydration sphere 13,13 19,9 13,4 shift to D

GuHCl 1.3 1.13 hydration sphere 13,13 19,9 13,4 shift to D

Solute	K_{P N}	K_{P D}	Select partition into	% surface polar backbone N,D	% surface charge N,D	% ΔSA N<=>D: backbone, charge	Effect on N<=> D
KCl	1	1	neither	13,13	19,9	13,4	none
urea	1.1	1.1	hydration sphere	13,13	19,9	13,4	shift to D
GuHCl	1.3	1.13	hydration sphere	13,13	19,9	13,4	shift to D