Calculated koff and t1/2 for binary complexes assuming diffusion-controlled kon

Complex

KD (M)

koff (s-1)

t

H2

1 x 10-71

1 x 10-63

2 x 1055 yr

RtV3 : Rt'L3(a)

10-17

1 x 10-9

2 yr

Avidin:biotin

10-15

1 x 10-7

80 days

thrombin:hirudin(b)

5 x10-14

5 x 10-6

2 days

lacrep:DNAoper(c)

1 x 10-13

1 x 10-5

0.8 days

Zif268:DNA(d)

10-11

1 x 10-3

700 s

GroEL:r-lactalbumin(e)

10-9

0.1

7 s

TBP:TATA(f)

2 x 10-9

2 x 10-1

3 s

TBP:TBP

4 x 10-9

4 x 10-1

2 s

LDH (pig): NADH(g)

7.1 x 10-7(j)

7.1 x 101

10 ms

profilin: CaATP-G-actin

1.2 x 10-6

1.2 x 102

6 ms

TBP: DNAnonspec(h)

5 x 10-6

5 x 102

1 ms

TCR(i): cyto C peptide

7X10-5

7X103

100 ms

lacrep:DNAnonspec(h)

1 x 10-4

1 X104

70 ms

uridine-3P: RNase

1.4 x 10-4 (j)

1.4X10-4

50 ms

Creatine Kinase: ADP

8.2 x 10-4 (j)

8.2X104

10 ms

Acetylcholine:Esterase

1.2 x 10-3

1.2 x 105

6 ms

no interaction

4 x 1073

4 x 1081

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  1. Trivalent Vancomycin derivative RtV3 + Trivalent D-Ala-D-Ala deriv, Rt'L3'

  2. Hirudin is a potent thrombin inhibitor from leach saliva

  3. lac rep is the E. Coli lac operon repressor protein, and DNAoper is the specific DNA binding region in the E. Coli genome that binds to the repressor

  4. Zif268 is a mouse zinc-finger binding protein

  5. GroEL is a chaperone protein; r-lactalbumin is the reduced form of lactalbumin

  6. TBP is the TATA Binding Protein which binds to the TATA box consensus sequence

  7. LDH is lactate dehydrogenase

  8. DNAnonspec is DNA which does not contain the specific DNA sequence region involved in specific
    binding to a DNA binding protein

  9. TCR is the T-cell receptor

  10. calculated from equation:  KD = koff/kon.