Calculated koff and t1/2 for binary complexes assuming diffusion-controlled kon
|
KD (M) |
koff (s-1) |
t ½ |
|
|
H2 |
1 x 10-71 |
1 x 10-63 |
2 x 1055 yr |
|
RtV3
: Rt'L3(a) |
10-17 |
1 x 10-9 |
2 yr |
|
Avidin:biotin |
10-15 |
1 x 10-7 |
80 days |
|
thrombin:hirudin(b) |
5
x10-14 |
5 x 10-6 |
2 days |
|
lacrep:DNAoper(c) |
1 x 10-13 |
1 x 10-5 |
0.8 days |
|
Zif268:DNA(d) |
10-11 |
1 x 10-3 |
700 s |
|
GroEL:r-lactalbumin(e) |
10-9 |
0.1 |
7 s |
|
TBP:TATA(f) |
2
x 10-9 |
2
x 10-1 |
3 s |
|
TBP:TBP |
4
x 10-9 |
4
x 10-1 |
2 s |
|
LDH
(pig): NADH(g) |
7.1 x 10-7(j) |
7.1 x 101 |
10 ms |
|
profilin: CaATP-G-actin |
1.2 x 10-6 |
1.2 x 102 |
6 ms |
|
TBP:
DNAnonspec(h) |
5
x 10-6 |
5 x 102 |
1 ms |
|
TCR(i): cyto C peptide |
7X10-5 |
7X103 |
100 ms |
|
lacrep:DNAnonspec(h) |
1
x 10-4 |
1 X104 |
70 ms |
|
uridine-3P: RNase |
1.4 x 10-4 (j) |
1.4X10-4 |
50 ms |
|
Creatine
Kinase: ADP |
8.2 x 10-4 (j) |
8.2X104 |
10 ms |
|
Acetylcholine:Esterase |
1.2
x 10-3 |
1.2
x 105 |
6 ms |
|
no interaction |
4 x 1073 |
4
x 1081 |
- |
Trivalent
Vancomycin derivative RtV3 + Trivalent D-Ala-D-Ala deriv, Rt'L3'
Hirudin is a potent thrombin inhibitor from leach saliva
lac rep is the E. Coli lac operon repressor protein, and DNAoper is the specific DNA binding region in the E. Coli genome that binds to the repressor
Zif268 is a mouse zinc-finger binding protein
GroEL is a chaperone protein; r-lactalbumin is the reduced form of lactalbumin
TBP is the TATA Binding Protein which binds to the TATA box consensus sequence
LDH is lactate dehydrogenase
DNAnonspec
is DNA which does not contain the specific DNA sequence region involved in
specific
binding to a DNA binding protein
TCR is the T-cell receptor
calculated from equation: KD = koff/kon.