| Km values | ||
| enzyme | substrate | Km (mM) |
| catalase | H2O2 | 25 |
| hexokinase (brain) | ATP | 0.4 |
| D-Glucose | 0.05 | |
| D-Fructose | 1.5 | |
| carbonic anhydrase | HCO3- | 9 |
| chymotrypsin | glycyltyrosinylglycine | 108 |
| N-benzoyltyrosinamide | 2.5 | |
| β-galactosidase | D-lactose | 4.0 |
| threonine dehydratase | L-Thr | 5.0 |
| kcat values | ||
| enzyme | substrate | kcat (s-1) |
| catalase | H2O2 | 40,000,000 |
| carbonic anhydrase | HCO3- | 400,000 |
| acetylcholinesterase | acetylcholine | 140,000 |
| b-lactamase | benzylpenicillin | 2,000 |
| fumarase | fumarate | 800 |
| RecA protein (ATPase) | ATP | 0.4 |