Questions
Fig 5 (not shown): The authors used UV-Vis and fluorescence spectroscopy to study the effect of NAC on the spectra of 1,4-naphthoquinone analogs. What likely effects would you expect to observe on the spectrum of lawsone?
Fig 6A: The ultimate goal of the investigators was to make a 1,4-naphthoquinone derivative that was not cytotoxic which presumably results from its interactions with free thiols. They synthesized PTK1 as shown below. Why would this reaction produce a potential inhibitor that did not react with free thiols in cells?
FIGURE 6BC. Investigators studied the effects of PTK1 on P300 acetylation of HeLa core histones with different concentrations of PTK1 in the absence and presence of NAC (Figure B) as well the presence of free thiol in the presence or absence of PTK1 (Figure C).
What can you conclude from these graphs?
Figure 6D: The figure below shows the UV-visible absorption spectrum of PTK1 in the in presence or absence of NAC?
What conclusions can you draw?
Fig 6E: The figure below shows immunoblotting analysis (IB) of cell lysates upon 24 h of treatment by PTK1 using H3K9 acetylation antibodies. The concentrations of PTK1 used are indicated in the figure E. Error bars, S.D. What conclusions can you draw from the blots?
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