48G7 Germline Fab Antibody Complexed With Hapten
5-(Para-Nitrophenyl Phosphonate)-Pentanoic Acid
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This antibody fragment is derived from an abzyme,
an antibody the possesses catalytic activity. In this case,
the antibody catalyses ester hydrolysis. The protein on the left depicts one arm of
an IgG antibody. Included are the variable light and heavy chains, and the constant light and constant 1 heavy chain. This can be seen in the circled portion of the
For more information see
Biochemistry Online: Chapter
7A: Methods of Catalysis
II. General Structure
Light Chain as Cartoon
Heavy Chain as Cartoon
Displays heavy chain in yellow and light chain in red.
Disulfide bridges, found between two
Cys amino acids, link the light chain with the heavy chain. Blue is the Heavy chain and Purple is the Light Chain.
A hapten is a small molecule which can bind to an antibody but is incapable of inducing an immunogenic response unless the hapten is attached to a carrier protein.
In this case, the hapten is a phosphonate,
a noncleavable transition state analog.
Hapten with Cartoon Heavy & Light Chains
The protein amino acids that bind to the hapten.
Wire Frame Ligand Bind Site
Hapten Ball & Stick / Binding Amino Acids Tubes