Biochemistry Online: An Approach Based on Chemical Logic

Biochemistry Online

CHAPTER 5 - BINDING

B:  MATHEMATICAL ANALYES OF BINDING GRAPHS

Last Updated: 3/25/16

Learning Goals/Objectives for Chapter 5B:  After class and this reading, students will be able to

  • derive equations  from the equation for fractional saturation  Y = ([ML]/[M0] = [L]/(KD+ [L])  equations for 1/Y as a function of 1/[L] (double reciprocal plot), Y/[L] vs Y (bound/free vs bound or the Scatchard Plot) and draw a plot of Y vs log [L] (semilog plot)
  • draw error bars on the plots of Y vs L, 1/Y vs I/L, Y/L vs Y and Y vs log L.
  • explain from these derivative equations and graphs how to calculate determine Kd
  • describe appropriate methods to fit the data from these equations with special attention given to the effect of error bars in the experimental value of Y

B1.  Binding of a Single Ligand

B2.  Binding of Two Identical Ligands

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