Learning Goals/Objectives for Chapter 5B:  After class and this reading, students will be able to

• derive equations  from the equation for fractional saturation  Y = ([ML]/[M0] = [L]/(KD+ [L])  equations for 1/Y as a function of 1/[L] (double reciprocal plot), Y/[L] vs Y (bound/free vs bound or the Scatchard Plot) and draw a plot of Y vs log [L] (semilog plot)
• draw error bars on the plots of Y vs L, 1/Y vs I/L, Y/L vs Y and Y vs log L.
• explain from these derivative equations and graphs how to calculate determine Kd
• describe appropriate methods to fit the data from these equations with special attention given to the effect of error bars in the experimental value of Y