Last Updated: 3/25/16
Learning Goals/Objectives for Chapter 5B: After class and this reading, students will be able to

Explore the interactive graphs below to see how binding of identical ligands to two
independent sites with different affinity changes the nature of the binding graphs. The
subtle changes in the graphs should make it clear that binding analyses
should be done with a rigorous mathematical analysis fitting of data to the
simplest hyperbolic model before moving on to more complex models.
Hyperbolic Graph for Two Independent Binding Sites
Wolfram Mathematica CDF Player  Fractional Saturation of Sites (01)for Binding of L to 2 Independet Sites  Hyperbolic Plot (free plugin required)
Double Reciprocal Graph for Two Binding Sites
Wolfram Mathematica CDF Player  Fraction Saturation of Sites (01) for Binding of L to 2 Sities  Double Reciprocal Plot. (Note that 1/Y max is 1 at the y intercept)
Scatchard Graph for Two Binding Sites
TBA
Wolfram Mathematica CDF Player  Semilog Plot for binding of ligand to 2 independent sites
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