CHAPTER 5 - BINDING
C: MODEL BINDING SYSTEMS
BIOCHEMISTRY - DR. JAKUBOWSKI
3/29/16
Learning Goals/Objectives for Chapter 5C: After class and
this reading, students will be able to
- explain the similarities and differences in structure
between myoglobin and hemoglobin in the deoxy and oxy states
- state structural features of Hb that stabilizes the
deoxystate and the oxystate
- draw graphs of fractional saturation Y vs L (or pO2) for Mb
and Hb (at different pHs and in the presence of CO2 for Hb) and
explain their apparent similarities and differences
- draw a thermodynamic cycle for the interactions of O2, CO2
and H+ with deoxy-Hb and oxy-Hb
- explain how Hill Plot analysis can account for cooperative
binding curves for Hb.
- give a simple explanation of the MWC model and draw cartoon
representations of Hb in the T and R state, describing the
characteristics of those states
- given definitions of the MWC parameters (L, KT, KR, c, and
α) and the assumptions of the model, explain how this model
accounts for cooperative sigmoidal binding curves for Hb and
dioxygen.
- draw cartoon models and explain differences in lock and key,
induced fit, and conformational selection as mechanisms for
ligand bind.
- Explain biological advantages elicited on ligand binding by
intrinsically disordered proteins
|
C5. Mathematical Analysis of Cooperative Binding - KNF Sequential
Model
This KNF (Koshland, Nemethy, and Filmer) Sequential Model:model was
developed to address concerns with the concerted model. One of the major
problems with the concerted model is that it seemed unrealistic to expect
all the subunits to change conformation together. Why shouldn't there be
some difference in subunit conformation? The KNF model also fits the
experimental data well.
Figure:
EQUILIBRIA IN KNF MODEL
Navigation
Return to
Chapter
5C:
Model Binding Sections
Return to
Biochemistry Online Table of Contents
Archived version of full Chapter 5C:
Model Binding Systems
Biochemistry Online by Henry Jakubowski is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.