Learning Goals/Objectives for Chapter 7B:  After class and this reading, students will be able to

• identify the types of catalysis used in enzyme-catalyzed reactions given a detailed mechanism;
• interpret kinetic experiments experiments varying substrate, inhibitors, pH, ion strength, and amino acid side chains (through chemical modification or site-specific mutagenesis) to better understand the catalytic mechanisms utilized in enzyme-catalyzed reactions;
• identify potential rate limiting steps in enzyme catalyzed reaction mechanisms and simplify kinetic equations based on them;
• generally describe the diversity, the critical active site residues and the biological activities of proteases;
• describe the structure/function of the proteasome.