Biochemistry Online: An Approach Based on Chemical Logic

Biochemistry Online


C: Dynamics of Membrane Lipids



Learning Goals/Objectives for Chapter 1C:  After class and this reading, students will be able to

  • state the different kinds of dynamical motions of double chain amphiphiles in lipid bilayers, differentiating between motion of entire molecules and motion within a molecule
  • describe experiments that could be used to study lateral and flip-flop diffusion in vitro and in vivo
  • explain the use of exogenous labels (fluorescent, radioactive, electron spin resonance) that allow laboratory determination of lipid location and movement
  • explain similarities and differences in measurement and explanations of phase transitions in water and in lipid vesicle
  • explain the biological effects and necessities of membrane dynamics

C6.  General Links and References

van Meer, G. et al.  Membrane lipids: where they are and how they behave. Nature Reviews Cell and Molecular Biology, 9, 112 (2008)

Andreyev, A.Y. et al.  Subcellular Organelle Lipidomics of TLR-4-Activated Macrophages.  Journal of Lipid Research 51, 2785 (2010).

Lingwood, D and Simons, K. Lipid Rafts as a Membrane-Organizing Principle.  Science 327, 46 (2010)

Baumgart, T. , Hess, S., and Webb, W. Imaging coexisting fluid domains in biomembrane models coupling curvature and line tension.  Nature. 425, pg 821 (2003)

Zacharias D. et al. Partitioning of Lipid-Modified Monomeric GFPs into Membrane Microdomains of Live Cells. Science. 296, pg 913 (2002)

van Meer, G. the Different Hues of Lipid Rafts. Science 296, pg 855 (2002)

Pike, Linda.  Lipid rafts: heterogeneity on the high seas.  Biochem. J. 378, 281–292 (2004)

Samsonov, A. et al. Characterization of Cholesterol-Sphingomyelin Domains and Their Dynamics in Bilayer Membranes.  Biophysical Journal 81, 1486–1500 (2001)

Simon & Ikonen.  How Cells Handle Cholesterol. Science. 290. pg 1709, 1721 (2000)

Azam et al. Membrane changes during hibernation. Nature. 407 pg 317 (2000)

Gatfield and Pieters.  Essential Role for Cholesterol in Entry of Mycobacteria into Macrophages.  Science. 288. pg 1647 (2000)

Borbat et al. Electron Spin Resonance in Studies of Membranes and Proteins. Science. 291. pg 266 (2001)


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