Learning Goals/Objectives for Chapter 6D:  After class and this reading, students will be able to

• draw Cleland chemical reaction diagrams showing enzyme, substrate, and product interactions for multisubstrate and multiproduct sequential and ping-pong enzyme-catalyzed reactions;
• draw double reciprocal 1/v vs 1/A plots at different fixed B concentrations for sequential and ping-pong multisubstrate reactions;
• draw v vs S graphs in the presence and absence of allosteric inhibitors and activators for multi-subunit enzymes that display sigmoidal cooperative behavior (K systems) conforming to the MWC model;
• differentiate between K and V systems for allosterically regulated enzymes using the MWC model and explain shifts in graphs of v vs S in the presence of allosteric effectors