Biochemistry Online: An Approach Based on Chemical Logic

Biochemistry Online

CHAPTER 6 - TRANSPORT AND KINETICS


D. MORE COMPLICATED ENZYMES

BIOCHEMISTRY - DR. JAKUBOWSKI

Last Update:  04/11/16

Learning Goals/Objectives for Chapter 6D:  After class and this reading, students will be able to

  • draw Cleland chemical reaction diagrams showing enzyme, substrate, and product interactions for multisubstrate and multiproduct sequential and ping-pong enzyme-catalyzed reactions;
  • draw double reciprocal 1/v vs 1/A plots at different fixed B concentrations for sequential and ping-pong multisubstrate reactions;
  • draw v vs S graphs in the presence and absence of allosteric inhibitors and activators for multi-subunit enzymes that display sigmoidal cooperative behavior (K systems) conforming to the MWC model;
  • differentiate between K and V systems for allosterically regulated enzymes using the MWC model and explain shifts in graphs of v vs S in the presence of allosteric effectors

D6.  References

  1. Pryciak, P. Customized Signaling Circuits.  Science 319, pg 1489 (2008)
  2. Bashor, C. Science 319, pg 1539 (2008)

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