Glyceraldehyde-3-phosphate dehydrogenase
Active site nucleophile Cys149Ser Mutant

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For more information see Biochemistry Online: Chapter 7C - Cofactors and Electron Pushing - Sources and Sinks

II. General Structure

Cartoon showing 4 monomers
Individual subunits with bound NAD+ (blue) and glyceraldehyde-3-phosphate  (green)
Active Site:NAD+ in Blue, Protein Wireframe; Cartoon Domains: NAD Binding Domain in Yellow, Substrate Binding Domain in Red
NAD+ (wireframe) Coordination Motif: Asp 32; Arg 72; Gly 97; Ser 119, 149 (His 176; Tyr 178; Asn 313
Spacefill visualization of bound substrates
Protein (gray), Binding Site Region (red), NAD+ (blue); Glyceraldehyde3-P (green)