BCHM321 Project Mechanism of auxin perception by the TIR1 ubiquitin ligase

Mechanism of auxin perception by the TIR1 ubiquitin ligase

HTML 5 version (does not require Java; downloads and moves slowly)

For more information see Biochemistry Online: Chapter 5D - Binding and the Control of Gene Transcription

Wire Frame in chains' colors
Three chains in the protein: Chain A - gray, Chain B - green, Chain C - red
Cartoon
Secondary structure of protein alpha helixes and beta sheets
Wireframe Auxin + Backbone Protein
Naphtalene-1-acetic acid (spacefill), a synthetic auxin (a mimetic of the most important auxin, indole acetic acid) and the backbone of protein
Temperature presentation of Protein with Auxin
The more mobile, "warmer" segments are colored red, progressing to more immobile blue fragments. NLA in spacefill.
Auxin interaction with protein
Chain B amino acids-green: HIS78, ARG403, SER438, SER462, GLU487, Chain C amino acids-red: PRO7, and TRP 5 interacting with NLA.
Solvent surface
Protein and Auxin with water (1.4 A) around the molecule.
MEP for protein
The Map of Electrostatic Potential (MEP) shows the charge distribution (d⁺and + charges which show as blue, and the d^- and - charges which show as red) on the atoms of the macromolecules. Black spacefill is NLA.

Tan, X., Calderon-Villalobos, L.I.A., Sharon, M.,Zheng, C.,Robinson, C.V., Estelle, M., Zheng, N. (2007) Mechanism of auxin perception by the TIR1 ubiquitin ligase Nature 446: 640-645