Structural basis of dsRNA processing by Dicer
Java version
HTML 5 version (does not require Java; downloads and moves
slowly)
For more information see
Biochemistry Online: Chapter 5D - Binding and the Control of Gene
Transcription
II. General Structure
Wireframe
Note: This tetrameric protein
structure does
not contain dsRNA.
Strands
Catalytic site
Note the GLU,
ASP interaction with the
Mn,
and the internal heterodimer formed by the two RNaselll located in
the head of each subunit.
Individual chains:
Individual dicer subunits of the tetramer will appear with time.
Wait until all show.
Secondary Structure
Alpha
Helicies &
Beta
Sheets.
Electrostatic map
Note the centralized positive charge density (blue) in
the PAZ regions used for binding of dsRNA
Amino
acids forming the 3' overhang-binding pockets
Located in the PAZ domain of the sub-units the highlighted
Tyrosine, Phenylalanine, and Histidine play
and important role in binding of dsRNA.