Biochemistry Online: An Approach Based on Chemical Logic

CHAPTER 9 - SIGNAL TRANSDUCTION

F: Signaling Math: Graphical Analyses of
Inputs and Outputs

Wimot Osei-Bonsu and Henry Jakubowski

11/16/17
New and slowly evolving .....

Learning Goals/Objectives for Chapter 9F: After class and this
reading, students will be able to

describe the steady state in context of an isolated single
enzyme-catalyzed reaction and for the enzyme when part of a
complex metabolic pathway in vivo;

state and describe what is required to perform a metabolic
control analysis on a biological pathway;

write ordinary differential equations to describe the change
in concentration of analytes in a given set of coupled
reactions;

write mass balance expressions for Eo and use it to
calculate the fraction of each enzyme form assuming rapid
equilibrium;

draw "wire" diagrams showing activation and inhibition for
coupled reactions;

define the flux control coefficient verbally and in the form
of a mathematic equation, and explain how to obtain it
graphically;

use flux control coefficients to explain how control of flux
in a pathway is distributed through all enzyme-catalyzed
reactions in that pathway;

analyze the result of a flux control analysis of a pathway
like glycolysis;

describe why small changes in the enzymes that have the
greatest ΔG values in glycolysis have minor effects in the
metabolic flux in glycolysis;

define the concentration control coefficient verbally and in
the form of a mathematic equation

analyze the result of a concentration control analysis of a pathway
like glycolysis;

define the elasticity coefficient verbally and in the form
form of a mathematic equation, and explain how to obtain it
graphically

state whether the different metabolic control coefficients
are global properties of the system or local properties of a
given enzyme.