Learning Goals/Objectives for Chapter 2H:  After class and this reading, students will be able to

• describe experimental evidence to show that protein misfolding and aggregation depends on the amino acid sequence and the environment in which folding occurs.
• describe conditions in vitro that may promote aggregation and how these might be minimized in vivo
• describe alternative conformations of prion proteins and relate them to a energy topology landscape
• explain how prion diseases may be transmitted in the absence of genetic material