CHAPTER 9  SIGNAL TRANSDUCTION
E: Metabolic Control Analysis and
Systems Biology
BIOCHEMISTRY  DR. JAKUBOWSKI
04/16/16
Learning Goals/Objectives for Chapter 9E: After class and this
reading, students will be able to
 describe the steady state in context of an isolated single
enzymecatalyzed reaction and for the enzyme when part of a
complex metabolic pathway in vivo;
 state and describe what is required to perform a metabolic
control analysis on a biological pathway;
 write ordinary differential equations to describe the change
in concentration of analytes in a given set of coupled
reactions;
 write mass balance expressions for Eo and use it to
calculate the fraction of each enzyme form assuming rapid
equilibrium;
 draw "wire" diagrams showing activation and inhibition for
coupled reactions;
 define the flux control coefficient verbally and in the form
of a mathematic equation, and explain how to obtain it
graphically;
 use flux control coefficients to explain how control of flux
in a pathway is distributed through all enzymecatalyzed
reactions in that pathway;
 analyze the result of a flux control analysis of a pathway
like glycolysis;
 describe why small changes in the enzymes that have the
greatest ΔG values in glycolysis have minor effects in the
metabolic flux in glycolysis;
 define the concentration control coefficient verbally and in
the form of a mathematic equation
 analyze the result of a concentration control analysis of a pathway
like glycolysis;
 define the elasticity coefficient verbally and in the form
form of a mathematic equation, and explain how to obtain it
graphically
 state whether the different metabolic control coefficients
are global properties of the system or local properties of a
given enzyme.

E1.
Introduction to Metabolic Control Analysis
E2.
Basic
Principles of Metabolic Control Analysis (MCA)
E3.
Metabolic Control Analysis and Simple Enzyme Inhibition
E4.
The Flux Control Coefficient
E5.
Concentration Control and Elasticity Coefficients
E6.
Links and References
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