Learning Goals/Objectives for Chapter 9E:
After class and this reading, students will be able to

• describe the steady state in context of an isolated single enzyme-catalyzed reaction and for the enzyme when part of a complex metabolic pathway in vivo;
• state and describe what is required to perform a metabolic control analysis on a biological pathway;
• write ordinary differential equations to describe the change in concentration of analytes in a given set of coupled reactions;
• write mass balance expressions for Eo and use it to calculate the fraction of each enzyme form assuming rapid equilibrium;
• draw "wire" diagrams showing activation and inhibition for coupled reactions;
• define the flux control coefficient verbally and in the form of a mathematic equation, and explain how to obtain it graphically;
• use flux control coefficients to explain how control of flux in a pathway is distributed through all enzyme-catalyzed reactions in that pathway;
• analyze the result of a flux control analysis of a pathway like glycolysis;
• describe why small changes in the enzymes that have the greatest ΔG values in glycolysis have minor effects in the metabolic flux in glycolysis;
• define the concentration control coefficient verbally and in the form of a mathematic equation
• analyze the result of a concentration control analysis of a pathway like glycolysis;
• define the elasticity coefficient verbally and in the form  form of a mathematic equation, and explain how to obtain it graphically
• state whether the different metabolic control coefficients are global properties of the system or local properties of a given enzyme.