Biochemistry Online: An Approach Based on Chemical Logic

Biochemistry Online

CHAPTER 6 - TRANSPORT AND KINETICS


C: ENZYME INHIBITION

BIOCHEMISTRY - DR. JAKUBOWSKI

06/12/2014

Learning Goals/Objectives for Chapter 6C:  After class and this reading, students will be able to

  • differentiate among competitive, uncompetitive, and mixed inhibition of enzymes by reversible, noncovalent inhibitors by writing coupled chemical equilibria equations and drawing cartoons showing molecular interactions among, E, S, and I;
  • using LeChatelier's principle and coupled chemical equilibria equations, draw double reciprocal (Lineweaver-Burk plots) and semilog plots for enzyme catalyzed reactions in the presence of different fixed concentrations of inhibitors and activators of enzyme
  • define KIS and KII for competitive, uncompetitive, and mixed inhibition from coupled chemical equilibria and double reciprocal plots;
  • differentiate between apparent and actual dissociations constants constants of an inhibitor and enzyme from double reciprocal plots and equations initial rate mathematical equations;
  • define agonist, partial agonist, antagonist, and mixed (noncompetitive antagonists) from analogy to enzymes and their inhibitors;
  • describe different ways that pH changes could affect the activity of an enzyme and suggest how each could affect Km and kcat. 

C1.  Irreversible Covalent Inhibition

C2. Competitive Inhibition

C3.  Uncompetitive Inhibition

C4. Noncompetitive and Mixed Inhibition

C5.  Enzyme Inhibition in Vivo

C6.  Agonist and Antagonist of Ligand Binding to Receptors - An Extension

C7.  Inhibition by Temperature  and pH Changes

C8.  Links and References

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