Biochemistry Online: An Approach Based on Chemical Logic

Biochemistry Online

 CHAPTER 6 - TRANSPORT AND KINETICS 

 
B:  Kinetics of Simple and Enzyme-Catalyzed Reactions

BIOCHEMISTRY - DR. JAKUBOWSKI

4/10/16

Learning Goals/Objectives for Chapter 6B:  After class and this reading, students will be able to

  • write appropriate chemical and differential equations for the rate of disappearance of reactants or appearance of products for 1st order, pseudo first order, second order, reversible first order reactions
  • draw and interpret graphs for integrated rate equations (showing reactant or product concentrations as a function of time) and initial rate equations (showing the initial velocity vo as a function of reactant ;
  • derive kinetic rate constants from data and graphs of integrated rate and initial rate equations;
  • write appropriate chemical, differential equations, and initial rate equations for the rate of disappearance of reactants or appearance of products for simple enzyme catalyzed reaction;
  • differentiate between rapid and steady state assumptions;
  • simplify the initial rate equation containing rate constants for an enzyme catalyzed reactions to one replacing the rate constants with kcat and KM, and give operational and mathematical definitions of those constants;

B1.  Single Step Reactions

B2.  Multi-Step Reactions

B3.  Rapid Equilibrium Enzyme-Catalyzed Reactions

B4.  Steady State Enzyme-Catalyzed Reactions  

B5.  Analysis of the General Michaelis-Menten Equation

B6.  More Complicated Enzyme-catalyzed Reactions

B7.  Meaning of Kinetic Constants

B8.  Experimental Determination of Vmax and Km  

 

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