Learning Goals/Objectives for Chapter 8B:  After class and this reading, students will be able to

• state the type of oxidizing reagent used and the products forms on oxidation reactions catalyzed by dehydrogenases, monoxygenases (hydroxylases), dioxygenases, and oxidases;
• draw the reactive end of NAD+ and mechanisms showing it's reactions with substrates in enzyme-catalyzed two electron oxidation reactions;
• explain differences in chemical reactivity of NAD+ and FAD in one and two electrons oxidations and with dioxygen;
• describe the stereochemistry of the alcohol dehydrogenase-catalyzed oxidation of prochiral ethanol by NAD+;
• explain why FAD/FADH2 are often tightly bound to dehydrogenases in contrast to NAD+/NADH where are freely diffusable substrates;
• given standard reduction potentials, determine the ΔGo' for given redox reactions;
• explain why different FAD and other flavin containing dehydrogenases have varying standard reduction potentials for the flavin but NAD+ dependent dehydrogenase have only one;
• describe the role of heme in mono- and dioxygenases in activating dioxygen and minimizing side reactions of ROSs;
• describe the biological role of cytochrome P450s;
• define and give examples of oxidases;
• compare the contrast the role of the heme in carrying hemoglobin and myoglobin, monoxygenases, and in oxidases.