Biochemistry Online: An Approach Based on Chemical Logic

Biochemistry Online





Learning Goals/Objectives for Chapter 8C: 

After class and this reading, students will be able to

  • explain reasons for the strongly exergonic hydrolysis of carboxylic acid anhydrides, phosphoric acid anhydrides, mixed anhydrides, and analogous structures and give approximate  values for the ΔG0 of hydrolysis of them;
  • identify from Lewis structures molecules whose hydrolytic cleavage are strongly exergonic;
  • explain how the exergonic cleavage of phophoanhydride bonds in ATP can be coupled to the endergonic synthesis of macromolecules like proteins;
  • draw mechanisms to show how oxidation and phosphorylation reactions are coupled in anaerobic metabolism through the productions of a mixed anhydride catalyzed by the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase;
  • explain how arsenate can double oxidation and phosphorlyation reactions in glycolysis
  • explain how NAD+ can be regenerated from NADH in anaeroboic condition to allow glycolysis to continue;
  • explain the general flow of electrons from NADH to dioxgen through a series of mobile and membrane protein bound electron acceptors in electron transport in the mitochondria inner member.
  • explain with picture diagrams how oxidation and phosphorylation reactions (to produce ATP) are coupled in aerobic metabolism through the generation and collapse of a proton gradient in the mitochondria;
  • draw pictures diagrams explaining the structure of F1F0ATPase in the inner mitochondria member and explain using the picture how ATP synthesis is coupled to protein gradient collapse
  • write an equation for the electrochemical potential and use it to calculate the available ΔG0 for ATP production on proton gradient collapse, given typical values for ΔpH and ΔE across the membrane 

C1.  ATP

C2.  Anerobic Coupling of Oxidation and ATP Synthesis

C3.  Aerobic Coupling of Oxidation and ATP Synthesis

C4.  An Overview of Mitochondrial Electron Transport

C5.  Complex I - NADH-quinone oxidoreductase

C6.  Complex III

C7.  Complex IV - Cytochrome C oxidase (CCOx)

C8.  Overall Coupling Oxidation and ATP Synthesis

C9.   ATP synthase

C10.  Proton Gradient Collapse and ATP synthesis - Structure

C11.  Proton Gradient Collapse and ATP synthesis - Thermodynamics

C12.   Metabolic Needs in Nonproliferating and Proliferating Cells

C13.  PPARs and the Regulation of Metabolism

C14.  Links and References


Return to Biochemistry Online Table of Contents

Archived version of full Chapter 8C:  ATP and Oxidative Phosphorylation


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Biochemistry Online by Henry Jakubowski is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.